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Fig. 6. Junctional ß-catenin and E-cadherin staining in SW480APC cells is disrupted by EGTA treatment and restored with excess Ca²⁺. MDCK (panels i-iii), SW480APC.15 (panels iv-vi) or SW480 (panels vii-ix) cells were stained with antibodies to ß-catenin (A), E-cadherin (B) or with Rhodamine-Phalloidin to visualize actin (C). Prior to staining, cells were serum starved overnight and left untreated (i, iv and vii), treated with 4 mM EGTA and 1 mM MgCl₂ for 1 our (ii, v, viii) or with 4 mM EGTA and 1 mM MgCl₂ for 1 hour followed by treatment with 10 mM Ca²⁺ for 2 hours (iii, vi, ix). Arrows indicate intact adherens junctions, and arrowheads indicate relocalized staining. Fluorescent staining was imaged by laser-scanning confocal microscopy. The images shown are for single-representative focal planes. Bar, 10 µm.

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