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Fig. 1. Anx2 overexpression in osteoblastic cells. SaOSLM2 cells were stably transfected with GFP or an anx2GFP fusion construct. (A) Immunoblot analysis confirmed that endogenous anx2 was expressed similarly in the GFP (1) and the anx2GFP (2) transfectants and the exogenous fusion protein was expressed in the transfectants. Endogenous anx2 was detected at approximately 36 kDa whereas the fusion protein was shifted to approximately 63 kDa because of the 27 kDa GFP tag. (B) Epifluorescence microscopy of GFP reveals that the fusion protein is excluded from the nucleus, similarly to endogenous anx2 protein expression (unpublished results) (200x).





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