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Fig. 8. Reduced anx2 expression in SaOSLM2 cells diminishes alkaline phosphatase activity and mineral crystal formation. (A) SaOSLM2 cells were transfected with an anx2-specific antisense oligonucleotide (1) or a control oligonucleotide (2). (B,C) Anx2 expression was analyzed by western blot at day 4. SaOSLM2 cells with either the anx2-specific oligonucleotide (Anx2AS) or the standard oligonucleotide (Std) were exposed to conditions to induce mineralization for 4 days. The cultures were stained with Alizarin Red S to detect deposition of mineral crystal (200x). (D) ALP activities were also measured in the Anx2AS (black) and the Std (white) oligonucleotide-treated cells.





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