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Fig. 2. Microtubule-dependent processes are defective in tbb-2 mutant embryos. (A) Nomarski differential interference contrast (DIC) micrographs of wild-type embryos beginning after the completion of meiosis II and ending during the first cytokinesis. In all panels in this and other figures, anterior is to the left and posterior to the right. m, maternal pronucleus; p, paternal pronucleus. Arrowheads mark positioning of the centrosomes during centrosome/pronuclear rotation and during anaphase (Movies 1-4, http://jcs.biologists.org/supplemental). (B) Centration in wild-type and tbb-2 mutant embryos. Each X represents the position along the long axis of the midpoint between the two centrosomes of the centrosome/pronuclear complex at nuclear envelope breakdown. If the pronuclei did not meet, the measurement was taken during nuclear envelope breakdown of the centrosome/paternal pronuclear complex. (C) Extent of centrosome/pronuclear complex rotation in wild type and in tbb-2 mutants, showing the angle of the mitotic spindle relative to the long axis just after nuclear envelope breakdown. (D) Anaphase spindle position in wild-type and in tbb-2 mutant embryos. Each bar represents the spindle position roughly 2 minutes after nuclear envelope breakdown in one embryo. Percentage of embryo length indicates the average spindle position relative to the anterior pole of the embryo.
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