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Fig. 3. Spindle microtubules and TBB-2 protein in wild-type and tbb-2 mutant embryos and extracts. (A) Western blot showing TBB-2 levels relative to an actin loading control, in embryo extracts from wild-type, gk129 and or362 animals (see Materials and Methods). or362 embryo extracts were prepared from worms matured at permissive or restrictive temperatures. (B) Western blot showing TBB-2 levels relative to an actin loading control in whole worm extracts prepared from wild-type and t1623 animals. (C) Indirect immunofluorescence images of wild-type and tbb-2 mutant embryos stained with antibodies that recognize
-tubulin (red) and TBB-2 (green); DNA was stained with TOTO (blue).