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Fig. 4. TBB-1 is upregulated in the absence of TBB-2. (A) Western blots showing TBB-1 levels in wild-type and tbb-2 embryo extracts that have reduced levels of TBB-2. Actin was used as an internal loading control. (B) Western blots showing TBB-1 levels in wild-type and tbb-2 (t1623) whole worm extracts, which have wild-type levels of TBB-2. (C) Fixed wild-type and mutant embryos stained with antibodies that recognize TBB-1 (green); DNA was stained with TOTO (blue). (D) Indirect immunofluorescence image of a fixed tbb-1(3' UTR RNAi) embryo stained with antibodies that recognize {alpha}-tubulin (red) and TBB-1 (green); DNA was stained with TOTO (blue).





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