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Fig. 4. Accumulation of YY1 in two distinct subcellular locations, cytoplasm and nucleus. Optical sections of triple-label images are shown for two cells fixed at different time points after shake-off (3 hours, A-C; 7.5 hours, D-F). Cells were stained by indirect immunofluorescence and data collected by serial optical sectioning microscopy. For each image, a projection (1-1.2 µm thick in Z comprising 4-6 optical sections) from a middle region of the data stack is shown. DAPI images (A,D) reveal the nucleus (boundary indicated by long-dashed lines). YY1 was visualized in the rhodamine channel (B,E), and BrdU was visualized in the FITC channel (C,F). The nuclear periphery (long-dashed lines) and the cell periphery (short-dashed lines) are indicated, and the nuclear and cytoplasmic areas are indicated by `n' and `c', respectively.





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