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Fig. 8. Cell-cycle changes in YY1 distribution observed by cofractionation assay. Cytoplasmic and nuclear extracts from synchronous populations of CHO cells were analyzed by western blot with anti-YY1 antibodies as described in Fig. 2B. Numbers above the lanes indicate the time of harvest (hours after shake-off). Cytoplasmic (Cyto.) or nuclear (Nucl.) extracts were loaded on an equal-protein basis (7 µg per lane).





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