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Fig. 2. Interaction between uPA, VN, suPAR and PN-1 in the solution phase. PN-1, VN, suPAR, uPA, PAI-1 and ATF (4 µg/ml each) were incubated in different combinations, as indicated, in TBS with 1% (w/v) BSA for 2 hours at 22°C. Thereafter, immunoprecipitation was performed with control mAb (lanes 1 and 8), anti-VN, 13H1 (lanes 2, 6 and 7), anti-uPAR, R4 (lane 5), anti-uPA, 4D1E8 (lane 4) or anti-PN-1, 4B3 (lanes 3 and 9) all at 4 µg/ml. The immune complexes were captured by adding protein A/G Sepharose and the immunoprecipitates were analyzed by western blotting with a rabbit polyclonal anti-uPAR followed by densitometric analysis. Similar results were obtained in three independent experiments.
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