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Fig. 3. Influence of PAI-1 on uPA-PN-1-induced VN binding to immobilized suPAR. Binding of VN (1 µg/ml) to suPAR-coated wells (5 µg/ml) was tested in the presence of increasing concentrations of PN-1 in the absence (solid triangle) or presence of uPA (20 nM; solid circle). For comparison, the binding of VN in the presence of uPA (20 nM) and PN-1 (250 nM) was determined in the presence of increasing concentrations of PAI-1 (open square). Binding was detected with the mAb VN-7, and data represent mean absorbance ±s.e.m. (n=3) of a typical experiment. Similar results were obtained in three separate experiments.
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