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Fig. 6. Localization of PN-1 in atherosclerotic vessels. Immunohistochemistry of arterosclerotic carotid artery, visualised through streptavidin-alkaline phosphatase conjugate, Fast Red stain and Mayer's hemalum counterstain. (A) General view (x34) of the endarterectomy specimen showing VN immunostaining. The extension of the neointima is indicated by a dotted line, the luminal border is marked by white arrows, the border of the neointima with the media is marked by black arrows. (B-F) The VN-positive foam cell accumulation, marked by a rectangle, shown at higher magnification. Serial sections (x272) showing PN-1 (B), uPAR (C), uPA (D), macrophage-antigen CD68 (E) and smooth muscle ${alpha}$ -actin (F) immunostaining. Note that PN-1 is expressed in VN-, uPAR- and uPA-positive areas (red stain). The foam cell accumulation is composed of smooth muscle cells and macrophages. Note the distribution of PN-1 that can be attributed to cytoplasmatic localization and matrix-associated protein. (G) A negative IgG control of PN-1 immunostaining of the same area (x272) was completely negative, underlining the specificity of the PN-1 immunoreactivity. (H) PN-1 was also demonstrated in a thrombus at the edge of a ruptured abdominal aortic aneurysm (x272). The dark arrows point to accumulations of PN-1-positive thrombocytes.

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