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Fig. 10. Effects of the expression of a stable version of Clb2. (A) Cells expressing Clb2{Delta}db arrested with a 2C DNA content. Flow cytometry analysis of wild-type and TAU58 cells grown in no-induction (CMD) and induction (CMA) conditions. Cells grown in CMD were centrifuged, washed twice in water, and resuspended in the appropriated medium. Samples were taken for FACS analysis at the indicated times. (B) TAU58 cells grown in CMA for 9 hours were stained with DAPI to observe nuclei. (C) TAU58 cells grown for 9 hours in CMD or CMA were stained with WGA to detect the presence of septum in the cells. Note that no septa are apparent in cells growing in CMA. (D) Formation of the mitotic spindle in TAU58 cells treated as before, observed with epifluorescence. Compare the MT pattern in non-induction conditions (CMD) and induction conditions (CMA). Scale bars: 5 µm.





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