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Fig. 6. Consequences of Clb2 overexpression. (A) Cells overexpressing a wild-type copy of Clb2 under the control of the P_crg1 promoter (TAU52-1 cells) were grown in CM with 2% arabinose for 9 hours and samples were DAPI stained (upper panel), or WGA stained to detect the presence of septa (lower panel). Scale bars: 10 µm. (B) Flow cytometry analysis of wild-type and TAU52-1 (FB1 Pcrg-clb2) cells grown in no induction (CMD) and induction (CMA) conditions. Cells grown in CMD were centrifuged, washed twice in water, and resuspended in the appropriated medium. Samples were taken for FACS analysis at the indicated times. The extra peak in TAU52 cells grown in CMA corresponds to a 4C DNA content. (C) Cell morphology of TAU26 (FB1 P_hsp70-clb2) cells growing in liquid CMD medium after 24 hours of incubation. Right panel show DAPI staining. Scale bar: 10 µm.

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