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Fig. 9. Effects of the expression of a stable version of Clb1. (A) Cell morphology of TAU57 strain grown in CMA for 9 hours. This strain carries the clb1{Delta}db1-2 transgene and a Cfp-tub1 fusion. All cells were arrested with a bud about the size of the mother cell. Three different condensed nucleus morphologies were apparent: a single nucleus near the neck, two nuclei nearby and a nucleus split in two located in the neck (arrows point to nuclei). Scale bar: 10 µm. (B) Cells expressing Clb1{Delta}db1-2 arrested with a 2C DNA content. Flow cytometry analysis of wild-type FB1 and TAU57 cells grown in no-induction (CMD) and induction (CMA) conditions. Cells grown in CMD were centrifuged, washed twice in water, and resuspended in the appropriated medium. Samples were taken for FACS analysis at the indicated times. (C) Microtubule organization in TAU57 cells growing in non-induction conditions (CMD) and induction conditions (CMA). Observe the absence of microtubule structures in inducing conditions.





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