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Fig. 2. Detection of overexpressed wt, 262A- and 262D-Cx43. (A) Western blot of lysates from vector-infected (10 µg/lane) or Cx43 (wt and mutant) overexpressing cultures (1 µg/lane; as indicated) probed with rabbit polyclonal anti-Cx43 antibodies. (B-D) Triple-fluorescence labelling, using rabbit polyclonal anti-Cx43 antibodies (green), mouse monoclonal anti-Cx43 antibodies (red) and Hoechst 33342 (blue). Myocyte cultures were infected with (B) vector, (C) 262A-Cx43 or (D) 262D-Cx43. Scale bar: 50 µm. Arrows point to areas of cell-cell contact.





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