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Fig. 3. Effect of wt or mutant Cx43 overexpression on cardiomyocyte anti-BrdU labelling. Myocyte cultures, infected with adenoviral vectors expressing wt-, 262A- and 262D-Cx43 (as indicated) were labelled simultaneously for BrdU (nucleus) and Cx43 (cell membrane), using monoclonal antibodies specific for each antigen. One colour is sufficient to detect both antigens, since they are found in different cellular locations. Arrows and arrowheads point to BrdU-positive and negative nuclei, respectively. Scale bar: 50 µm.





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