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Fig. 2. Graphs to show the uptake of radiolabeled AdoMet (S-adenosyl-L-[methyl-3H]methionine) by D amoebae (A) and xD amoebae (B). S-Adenosyl-L-[methyl-3H]methionine was added to 2.0x103 amoebae/ml in Chalkley's solution to a final concentration of 2 µCi/ml. Total uptake ({bullet}) was determined from collected amoebae before sonication. After sonication and centrifugation, incorporated AdoMet into macromolecules ({circ}) was estimated from the pellets, and net transport ({blacktriangleup}) from supernatants. Results are expressed as percentage of the initial radioactivity of AdoMet. Values are averages from two separate experiments.





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