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Fig. 4. Fractionation of SAMS from D and xD ameobae. (A) Results of Sephadex G-150 chromatography of D and xD amoebae. (B) Results of DEAE-cellulose chromatography of D and xD amoebae. Cytosolic extracts (2-3 ml) of D and xD ameobae were chromatographed, and 25 µl aliquot from each fraction was used in the assay of SAMS activity. The Sephadex G-150 column was previously calibrated with the following molecular markers (arrows): a, catalase (232 kDa); b, aldolase (158 kDa) and c, albumin (67 kDa).





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