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Fig. 5. Results of western blot analyses of proteins fractionated by Sephadex G-150 chromatography. Fractions containing SAMS enzyme activities (Fig. 4) were subjected to western blotting using pAb against amoeba SAMS1. A 15 µl aliquot from each fraction was separated by SDS-PAGE (10%), and transferred to a membrane for western blotting. Whole-cell proteins of D amoebae were loaded in the first lane as a size marker for amoeba SAMS1.





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