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Fig. 3. Electron microscopic appearance of wild-type and swm1 mutant cells incubated for 12 hours at 38°C. (A,C) Wild-type strain YPA24; (B,D-F) swm1 strain YPA207. Cells grown in YEPD supplemented with 1 M sorbitol are shown at low magnification in (A,B), whereas higher magnifications of the septal region are shown in (C-F). The structure of the septum is clearly visible in wild-type cells, in which the chitin-rich primary septum (ps) is surrounded by the secondary septum (ss), composed mainly of glucans and mannoproteins. The chitin ring (cr) synthesized by Chs3p is visible at both sides of the primary septum. In mutant cells (D-F), the same structure is visible but the region corresponding to secondary septum (ss) is thickened. Scale bars: 1 µm (A,B), 0.5 µm (C-F).





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