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Fig. 6. Cells with the swm1 mutation have a defect in exit from mitosis. Cultures of isogenic wild-type (W9317) or swm1 mutant (SEP3) cells containing an epitope-tagged version of the CLB2 gene (CLB2^HA) were arrested in mitosis with nocodazole (2.5 hours) before transfer to the restrictive temperature for the swm1 mutation (38°C, 1.5 hours). Cultures were then released from nocodazole arrest and incubated at 38°C. Samples were taken at the indicated intervals after the release (minutes) and processed for FACS analysis (A), Clb2 and Pgk1 western analysis (B) or Clb2-associated H1 kinase activity assays (C). In each panel asynchronous cultures (A) are also shown for reference.

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