QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 2. Sec6 co-localizes with components of the nectin complex in early cell-cell contacts and polarized MDCK cells. (Top panels) Low-density cultures of MDCK cells were allowed to form calcium-dependent cell-cell contacts for 1 hour, and then fixed with 2% paraformaldehyde before extraction with 1% Triton X-100. Sec6 distribution was compared to that of afadin or nectin-2 ${alpha}$ . (Bottom panel) Confluent MDCK cultures on polycarbonate filters were fixed and extracted 24 hours after induction of calcium-dependent cell-cell adhesion. Sec6 distribution was compared to that of afadin. Anti-Sec6 monoclonal antibody (9H5) was visualized with FITC-labeled goat anti-mouse antibody (in the afadin panels) or with Texas Red-labeled donkey anti-mouse antibody (in the nectin panel). Rabbit polyclonal antibody to afadin was visualized with Texas Red-labeled donkey anti-rabbit antibody, and rat monoclonal antibody to nectin-2 ${alpha}$ was visualized with FITC-labeled goat anti-rat antibody. Confocal images were obtained as described in Fig. 1 legend. Scale bar: 10 µm.

Return to article