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Fig. 8. E-cadherin and nectin-2
cooperate to recruit Sec6/8 complex to intercellular contacts in fibroblasts. LE cells, or LE cells that had been transiently transfected with pcDNA3.1-IgK-2HA-nectin-2, were cultured without (no Dex) or with (plus Dex) 10-6 M dexamethasone for 18 hours to induce E-cadherin expression. Cells were fixed with 4% paraformaldehyde and then extracted with buffer containing 1% Triton X-100. Anti-Sec8 monoclonal antibody (2E9) was visualized either with FITC- or Texas Red-labeled secondary antibodies. Anti-E-cadherin polyclonal antibody (UVO) was visualized with Texas Red-labeled secondary antibody. Anti-nectin-2 rat monoclonal antibody was visualized with FITC-labeled secondary antibody. Arrows indicate homotypic cell-cell contacts formed between adjacent cells expressing either nectin-2 or E-cadherin only. Arrowheads indicate cell-cell contacts between E-cadherin-expressing cells in which nectin-2 was also expressed. Scale bar: 30 µm.
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