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Fig. 6. Identification of endogenous tenascin-W. (A) Western blot analysis with an anti-tenascin-W antibody of extracts from E10.5 and E12.5 whole embryos, intestine and liver of E18.5 embryos, periosteum and kidney of adult mice and of embryonic stem (ES) cells conditioned medium. All of the samples reveal a single tenascin-W band of 180 kDa except for liver, which is negative for tenascin-W. (B) Agarose gel of nested-PCR products amplified from an E18.5 whole embryo mouse library using primers covering the second to the ninth fibronectin type III repeats of mouse TN-W, with (a) and without (b) template.





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