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Fig. 7. C2C12 osteoblasts express tenascin-W and adhere to a tenascin-W coated substratum. (A) Tenascin-W accumulates in the conditioned medium of BMP2-treated C2C12 cells (+) but not in untreated cultures (-) collected after 2, 4 and 6 days of treatment as revealed on the immunoblot with anti-tenascin-W antibody. The C2C12-produced tenascin-W migrates with the purified recombinant tenascin-W loaded as control (TNW). (B) Adhesion assay of C2C12 cells after culturing for 6 days with or without BMP2 on fibronectin. (C) Adhesion assay of C2C12 cells after culturing for 6 days with or without BMP2 on tenascin-W. Treated and untreated cells adhered equally well to fibronectin but BMP2 treatment increased the adhesivity of the cells to tenascin-W. (D,E) Photographs of the C2C12 cells of the adhesion assay shown in (B,C). Cells without BMP treatment on fibronectin (D), on tenascin-W (E) and BMP2-treated cells on tenascin-W (F). The cell morphology of treated and untreated cells remains the same. Scale bar, 20 µm (D-F).





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