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Fig. 9. Overexpression of 8 integrin increases adhesion of C2C12 cells to tenascin-W. Phalloidin staining of C2C12 cells after 1 hour of adhesion on fibronectin (A) reveals stress fibre formation. Phalloidin staining of C2C12 cells reveals the extension of actin-rich processes in cells adherent to tenascin-W (B). C2C12 cells were transfected with an 8 expression plasmid alone (C,D) together with an EGFP plasmid (E-G) or with the EGFP plasmid alone (H-J) and, 2 days later, replated for 1 hour on tenascin-W-coated wells. After fixation, cells shown in (C,D) were stained with anti-8 antiserum (C) and phalloidin (D). Cells overexpressing 8 are more spread and contain stress fibres, whereas the untransfected cell to the right (arrow) starts to make actin-rich processes. Cells transfected with 8 are more spread and have longer processes (E-G) than EGFP-only transfected cells (H-J). Scale bar, 25 µm (A,B,E-J), 10 µm (C,D).

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