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Fig. 6. Stimulation of cells with either isoproterenol or forskolin provokes microtubule reorganization. Untreated A431 cells (control, -), or cells pretreated with either nocodazole or taxol (see legends to Figs 1 and 3) were stimulated with either isoproterenol (10 µM, +Iso) for 30 minutes or with the plant diterpene forskolin (100 µM, +Forskolin) for 15 minutes. After the stimulation, the cells were washed, fixed and immunostained to visualize microtubules (see legend to Fig. 3). Stimulation by isoproterenol induces changes in the organization of microtubules, i.e. the radiance of microtubules from the central regions to the periphery of the cell was more prominent and multiple nucleation centers (note in the forskolin-treated cells) appeared. Treatment either with nocodazole or taxol perturbs these patterns of microtubule organization, i.e. radiating microtubule arrays were absent. Scale bar, 10 µm.
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