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Fig. 2. Deletion analysis of the Rad9 N-terminus. (A) Phenotypes of truncation and internal deletion RAD9 alleles in two growth assays. A series of N-terminal truncations and internal deletions on single copy plasmids transformed into a rad9{Delta} cdc13-1 strain. The gaps in the black bars represent in-frame deletions of the coding sequence. Numbers over the black bars correspond to the amino acids that remain flanking the deletions. Data is not shown for constructs rad9{Delta}1-159, rad9{Delta}1-199, rad9{Delta}1-239, rad9{Delta}40-159, rad9{Delta}80-119, rad9{Delta}80-159, rad9{Delta}80-231 or rad9{Delta}120-159, all of which had intermediate-strength rad9s phenotypes similar to those shown in rows 4-6 and 8-10. (B) The rad9{Delta}1-231 allele has the strongest rad9s phenotype when integrated; de novo deletion alleles of RAD9 were integrated at the URA3 locus of a rad9::LEU2 cdc13-1 cdc15-2 strain and tested in the two growth assays.





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