JCS00879 Movies

Files in this Data Supplement:

• Movie 1 -

  Movie 1A2 in culture shows a time-lapse movie of the normal spreading and interaction of control 1A2 NIH3T3 cells in the presence of mifepristone.

• Movie 2 -

  Movie 2D3 in culture shows a time-lapse movie of the substrate adhesion and movement of mifepristone-treated, DEP-1-expressing 2D3 NIH3T3 cells. These cells show aberrant substrate attachment, spreading and movement.

• Movie 3 -

  Movie 1A2 in PDGF shows a time-lapse movie of a representative control mifepristone-treated 1A2 NIH3T3 cell in the presence of a gradient of PDGF. The cell displays directed locomotion up the gradient toward the source of PDGF.

• Movie 4 -

  Movie 2D3 in PDGF shows a time-lapse movie of a representative mifepristone-treated, DEP-1 expressing  2D3 NIH3T3 cell in a gradient of PDGF. The cell displays random motility and does not recognise nor move up the concentration gradient of PDGF.

• Movie 5 -

  Movie 1A2 IRM leading edge shows a time lapse movie using interference reflection optics of a control 1A2 NIH3T3 cell in the presence of mifepristone. The cell shows the normal development and maturation of distinct focal adhesions starting at the leading edge and maturing into arrowhead-like or elongated focal contacts.

• Movie 6 -

  Movie 2D3 IRM leading edge shows a time lapse movie using interference reflection optics of a DEP-1-expressing 2D3 NIH3T3 cell in the presence of mifepristone. The cell shows smaller focal adhesions at the leading edge which do not mature into larger elongated substratum contacts.