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Fig. 5. DEP-1-expressing cells exhibit defective motility and interaction with fibronectin. (A) Parallel cultures of 1A2 and 2D3 cells imaged at 1-hour intervals over 6 hours showing lack of cell spreading in DEP-1-expressing 2D3 cells. (B) Quantification of cell adhesion and spreading on a fibronectin substratum. Cells were plated on to fibronectin-coated coverslips and washed after either 1 hour or 3 hours, as indicated, before fixation and counting. (C) Tyrosine phosphorylation of FAK. Cells were plated on fibronectin (FN plating +) or held in suspension (FN plating -). Lysates were immunoprecipitated with anti-FAK antibody followed by immunoblotting with anti-phosphotyrosine (upper panel) or ant-FAK (lower panel).





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