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Fig. 3. Localization of Syne-1 during mitotic cell division. Mitotic cells were fixed and stained with anti-tubulin (green), polyclonal anti-Syne-1 (red) and DAPI (blue). Localization of Syne-1 during various stages of mitosis (a,c,e,g,i). Triple-staining of the same cells indicate the localization of Syne-1 relative to the mitotic spindle and chromosomes (b,d,f,h,j). At stages before and during metaphase (a-d) Syne-1 is localized at punctate structures typical for Golgi markers (a-d, arrows). After metaphase Syne-1 is localized at a band of material that bisects the central spindle (e-j). This localization persists through cytokinesis where midbody localization is observed (i and j).





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