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Fig. 5. Association of Syne-1 and kinesin II with central spindle and midbody is detergent resistant. NRK cells were extracted with Triton X-100 before fixation and then stained with anti-Syne-1 antibody (red) (a,b), for KIF3B with anti-kinesin II antibody (red) (c,d) and DAPI (blue). Both Syne-1 and kinesin II localized to detergent insoluble structures associated with the central spindle in anaphase (arrow) (a,c) and the midbody during cytokinesis (arrow) (b,d).





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