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Fig. 6. Association of kinesin II and Syne-1 with the midbody is sensitive to ATP. To determine whether Syne-1 and kinesin II interactions with the mitotic spindle were coupled, distribution of Syne-1 (g,i,k) and kinesin II (a,c,e) was investigated in cells that had been detergent extracted in the presence of 10 mM MgSO4 (a,b,g,h), 10 mM MgATP (c,d,i,j) or 10mM of a non-hydrolyzable ATP anolog AMP-PNP (e,f,k,l). Cells were stained with antibodies against KIF3B (a,c,e) or Syne-1 (g,i,k) and then double-stained with anti-tubulin antibody (b,d,f,h,j,l) to visualize the mitotic spindle. Localization of both kinesin II and Syne-1 are greatly reduced in the presence of ATP (indicated by arrowheads) (c,i) and slightly enhanced in the presence of AMP-PNP (indicated by arrows) (e,k).





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