spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 8. Syntaxin localizes to the central spindle and midbody during anphase and cytokinesis. NRK cells in late stages of mitosis were fixed and stained with antibodies against syntaxin (a,e,f,g) protein disulfide isomerase (PDI) (b), E-cadherin (c). Cells stained for cadherin were also double stained with anti-Syne-1 to visualize the central spindle (arrow) (d). Syntaxin, concentrates in the midbody during cytokinesis (a,e,g), similar to Syne-1 and kinesin II. By contrast, accumulation within the midbody was not observed for PDI (arrow) (b) or E-caherin (arrow) (c). (e-h) Cells extracted with TX-100 before fixation with (f) or without (e) ATP, or with non-hydrolyzable ATP analog AMP-PNP (g,h). Cells treated AMP-PNP were double stained with anti-syntaxin (g) and anti-tubulin (h) antibodies. Notice that, as we have observed with KIF3B and Syne-1, syntaxin association with the midbody is resistant to TX-100 extraction (e, arrow), greatly reduced in the presence of ATP (f, arrow), and stabilized in the presence of non-hydrolyzable ATP (g, arrow)





Right arrow Return to article