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Files in this Data Supplement:
Movie 1. Time-lapse fluorescent microscopy reveals the accumulation and dynamics of GFP-myosin in post-mitotic C2C12 myocytes. Two different cells from the same field are shown in 100 frames taken 10 minutes apart over the 16 hours 30 minutes duration of the time-lapse experiment. Cells were infected 24 hours before the start of observation. Left: The GFP-myosin is first detected near the cell boundary around the nucleus (N) in the globular intermediates. The fluorescence intensity and number of globules increases with time and the globules move about the cytoplasm as this mono-nucleated myocyte elongates. The globules are pulled along with the elongating cell, forming a fiber that stretches 25-30 µm (the arrows heads in last image). Right: Another cell elongates and with the elongation there is a re-distribution of the globular intermediates to the very tip of the cytoplasmic extension and rapid movement of the fluorescent fibrils throughout the elongated cytoplasm. The arrowhead marks the approximate end of the extended myocyte. (See Fig. 2).
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