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Fig. 5. Immunofluorescence analysis of COS-7 cells not transfected or transfected with WT rNIS or rQ267E NIS. Non-permeabilized (A,C) or permeabilized (B,D) transfected COS-7 cells were incubated with 2 nM anti-Ct-rNIS Ab followed by fluorescein-conjugated goat anti-rabbit Ab. The rectangular panels above B and D show xz cross-sectional images obtained using a confocal microscope





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