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Fig. 7. Effect of charged side-chain Q267 substitutions on NIS expression. (A) Non-transfected COS-7 cells (NT) or COS-7 cells transfected with either WT rNIS or rQ267D, rQ267K or rQ267R NIS were assayed for I- transport activity at 20 µM I- at steady-state. Assays were performed in the presence of 20 µM I-/140 mM Na+ (colored bars) or 20 µM I-/140 mM Na+ plus 80 µM perchlorate (white bars). Results are expressed as a percentage of WT NIS activity. The y-axis has been split into two scales. (B) Immunoblot. (C) Surface biotinylation. (D) Immunofluorescence analysis. Procedures were performed as described in Figs 3, 5 and 6, and in Materials and Methods.





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