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Fig. 9. Tea1p shows a non-uniform association with microtubules in mod5{Delta} tea2-1 mutants before cell branching. Anti-tubulin (A,D) and anti-tea1p (B,E) immunofluorescence, and merged images (C,F) in tea2-1 (A-C) and mod5{Delta} tea2-1 (D-F) mutants 60 minutes after temperature shift to 36°C. Note that, in mod5{Delta} tea2-1 cells, tea1p often appears more concentrated on microtubules towards the cell middle (yellow in merged panel F), relative to the total microtubule signal (arrows in D-F). Also shown are merged images of branching mod5{Delta} tea2-1 mutants 90 minutes after temperature shift (H), as well as wild-type cells without a temperature shift (G), to show the relatively low tea1p signal in these mutants as compared with wild-type cells.





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