spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 1. The subcellular localization of mSTI1-EGFP and NLSmSTI1-EGFP. (A) NIH3T3 fibroblasts were transiently transfected with pB-mSTI1-EGFP, fixed in 3.7% paraformaldehyde, permeabilized in 0.1% saponin (all panels) and immunostained with SF1 polyclonal rabbit anti-mSTI1 and Cy3-conjugated donkey anti-rabbit antibodies (middle and right panel). Cells were mounted and visualized by confocal laser fluorescence microscopy. Scale bars, 10 µm. (B) NIH3T3 fibroblasts were stably transfected with pB-EGFP (top), pB-mSTI1-EGFP (middle) and pB-NLSmSTI1-EGFP (bottom). Cells were fixed in 3.7% paraformaldehyde and stained with DAPI before visualization by confocal laser fluorescence microscopy. Scale bars, 10 µm.





Right arrow Return to article