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Fig. 2. Co-immunoprecipitation of Gea2p and Drs2p from yeast cells. C13-ABYS86 cells carrying 2µ-DRS2 (pRS425-DRS2) and either 2µ-GEA2 (pGMS3) or 2µGEA2-HA (pGMS3-HA) were grown to exponential phase and lysates prepared. Gea2p-HA was immunoprecipitated using anti-HA antibodies, and the bound material run on an SDS-polyacrylimide gel and transferred to nitrocellulose. Antibodies against Drs2p were used to probe the blots.





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