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Fig. 6. The gea2-V698G mutant fails to interact with Drs2p, and is temperature and BFA-sensitive. (A) Strain Y190 carrying the C-terminal region of Drs2p (amino acids 1230-1355) in the two-hybrid prey plasmid and either the wild-type Gea2 or gea2-V698G mutant Sec7 domain as the bait plasmid were grown on medium containing X-gal (left) or on control medium (right). (B) Strains CJY092 gea1{Delta} gea2{Delta}/pSKP1 (pCEN-GEA2) and CJY093 gea1{Delta} gea2{Delta}/pCEN-gea2-V698G were grown to stationary phase and serial dilutions spotted onto plates grown either at 30°C or 37°C. (C) Strains CJY092 gea1{Delta} gea2{Delta}/pSKP1(pCEN-GEA2) and CJY093 gea1{Delta} gea2{Delta}/pCEN-gea2-V698G were grown to stationary phase and serial dilutions spotted onto plates containing 30 or 50 µg/ml BFA or solvent alone (methanol). Plates were incubated at 30°C.





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