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Fig. 8. Ultrastructural analysis of gea2-V698G and control cells grown to exponential phase at 24°C, then shifted for 1 hour to 38°C. Stereopairs of 0.20 µm thick sections of (A,B) CJY092 gea1 ${Delta}$ gea2 ${Delta}$ /pCEN-GEA2 and (C-F) CJY093 gea1 ${Delta}$ gea2 ${Delta}$ /pCEN-gea2-V698G cells. ER, endoplasmic reticulum, G, Golgi, V, vacuole, SG, secretion granule/vesicle. (A) An image showing typical Golgi tubular network structures (G) scattered throughout the cytoplasm. (B) A cell with an emerging bud (lower right) with a typical accumulation of homogeneously sized secretion granules/vesicles (SG). (C) Cell showing abnormal accumulation of membranes throughout the cell. (D) Cell with an emerging bud lacking the normal accumulation of secretion granules/vesicles. Note the heterogeneous size and scattered distribution of secretion granules/vesicles (arrowheads). To view the 3D image of the structures, figures should be viewed through a pair of anaglyph (red-cyan) glasses with the red lens placed over the left eye. Scale bars: 0.5 µm.

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