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Fig. 9. (A,B) Stereopairs of 0.20 µm thick sections of CJY093 gea1{Delta} gea2{Delta}/pCEN-gea2-V698G cells prepared as described in Fig. 8. (A) Cell showing an abnormal Golgi element (arrow) with heterogeneously sized fenestrations and segregating granules (one indicated with an arrowhead). (B) Cell showing an abnormal Golgi element with almost no fenestrations (arrow) but nevertheless containing a segregating granule (arrowhead). (C-E) Strain SEY6210 drs2{Delta}URA3 was grown to exponential phase at 30°C, then shifted to 24°C for 1 hour. Stereopairs of 0.20 µm thick sections are shown. (C) Cell showing accumulation of spherical membrane structures (S) made up of unfenestrated membrane. (D) A higher magnification image of a spherical element made up of abnormally fenestrated membrane (white arrowhead). The element is connected to a ribbon of ER that, on the right, is continuous with the nuclear envelope. (E) Cell showing an abnormal Golgi element with almost no fenestrations (arrow) and containing segregating granules (one is marked by an arrowhead). ER, endoplasmic reticulum; G, Golgi; V, vacuole; SG, secretion granule/vesicle; S, spherical structure. To view the 3D image of the structures, figures should be viewed through a pair of anaglyph (red-cyan) glasses with the red lens placed over the left eye. Scale bars: 0.5 µm.





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