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Fig. 2. Effect of arrestins and dominant-negative mutants of arrestin-2 and dynamin on agonist-mediated internalization of ß-subtypes. Cells were transiently cotransfected with equal amounts of total plasmid DNA containing Zem228c-ß1AR or -ß2AR, and either pcDNA3 (con), pcDNA3-arrestin-2 (arr-2), -arrestin-3 (arr-3), -arrestin-2-(319-418) (DN-arr), or -dynamin-K44A (DN-dyn). After 48 hours, the cells were incubated for 30 minutes with or without 1 µM ISO and assayed for surface receptors. Results represent the means±s.e.m. of 3-6 experiments for each condition. Although ßAR expression levels varied, they were similar for both subtypes at each condition.





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