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Fig. 2. Defects in the surface organization of laminin and dystroglycan in dy2J muscle. Confocal images of teased dy2J dystrophic muscle fibers (b-e: b, merged image; c, laminin-{alpha}2-G; d, {alpha}-dystroglycan). A wild-type control fibril (merged image) is shown in a. (e) A deconvoluted widefield cross-sectional view of dystrophic basement membrane zone (dystroglycan, red; laminin, green; merge, yellow). Note extensive (but not complete) effacement of rectilinear distributions for surface-distributed basement membrane, receptor and cytoskeletal components. Bars, 2 µm in e.





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