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Fig. 4. Grading of normal and dy2J surface architectures. Three classes of surface architecture were defined from the dystroglycan immunofluorescence patterns. Class 1, seen in normal muscle, corresponds to a regular rectilinear pattern. Class 2, largely present in dystrophic muscle, consists of a less regular lattice with splaying and with frequent loss of the finer circumferential and longitudinal lattice struts. Class 3 is a dystrophic pattern characterized by substantial loss of the rectilinear pattern with extensive inter-array clearing (shown) or near-complete pattern effacement. Corresponding paired images with 1.5x magnified insets for dystroglycan shown.





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