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Fig. 1. CgA and CgB levels in clones isolated from wild-type PC12 (A) and in subclones isolated from the PC12-15 clone after stable transfection of empty vector (A) or antisense CgA sequences (B). Scatter plot analysis of CgA and CgB western blot bands decorated by the specific polyclonal antisera, GE-19 and PE-11, respectively. The gels were loaded (25 µg of protein/lane) with total cell lysates. The densitometric CgA and CgB signals, normalised to tubulin, are indicated as percentages of the values in the reference PC12-15 clone. (A) Open squares illustrate the CgA/CgB ratios of 9 PC12 clones isolated from wild-type PC12 (Clementi et al., 1992); closed squares the ratios of 3 subclones isolated from the PC12-15 clone after transfection with the empty vector. (B) Twelve subclones of the reference PC12-15 clone, isolated after stable transfection with antisense sequences against rat CgA. A,L,O are the subclones illustrated in the corresponding panels of Fig. 2.





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