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Fig. 2. Distribution of CgA and CgB in the cells of subclones isolated from the PC12-15 clone after stable transfection with CgA antisense sequences. The confocal images of CgA (GE-19 antiserum, red) are shown in the left panels; those of CgB (monoclonal CIRO, green) are in the middle; the merged images are to the right. Subclone V was transfected with the empty vector; V' is a close-up view of cells showing non-parallel distribution of CgA and CgB in cytoplasmic granules or groups of them. A,L,O were transfected with CgA antisense sequences. Arrows indicate cells very low in CgA where CgB is in contrast at control levels or above. A cell with CgA levels similar to controls and poor in CgB is indicated by the short arrow. Scale bar: 8 µm.
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