QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. Nature of proteins in inefficiently GPI-processed CC1 mutant, CC1-t. (a) Immunoblot of the supernatant of LR(CC1-t) transfectants with and without PI-PLC treatment. PI-PLC treatment released only one band (lane 1), which corresponds to the TX-100-insoluble, higher M_r band (lane 3). Two other low M_r bands, which could be seen in both PI-PLC-treated and untreated cells (lane 1 and 2) but not in TX-100 extracts (lane 3 and 4), are presumably the result of other cross-reacting components in the reaction medium (see Materials and Methods). (b) Immunoblot of both TX-100-soluble and -insoluble fractions with (lanes 3 and 4) and without (lanes 1 and 2) treatment with Endo H. Only the major lower M_r, TX-100-soluble band (lane 3) was sensitive to Endo H digestion.

Return to article