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Fig. 5. Homotypic intercellular adhesion mediated by mutant GPI-anchored CEACAM1 protein. CC1-tAT transfectants of LR-73 cells were subjected to the homotypic adhesion assay in suspension. The percentage of single cells was measured as a function of time in suspension. Neo (vector alone) and CC1-4L transfectant cells were used as negative and positive controls, respectively. The mean expression levels of CC1-4L and CC1-tAT by FACS analysis were 280 and 98 fluorescence units, respectively.